Influence of the phytoestrogens of Sambuccus nigra and Petroselinum crispum on the proliferation and receptor expression in breast cancer cell lines

Schröder, Lennard (2021): Influence of the phytoestrogens of Sambuccus nigra and Petroselinum crispum on the proliferation and receptor expression in breast cancer cell lines. Dissertation, LMU München: Medizinische Fakultät

Abstract

Laboratory and clinical research on phytoestrogens suggests related dietary health benefits and relevant pharmaceutical implications for the treatment of breast cancer. Phytoestrogens are a group of polyphenols produced by plants that functionally and structurally resemble 17β-estradiol and can therefore interact as agonists or antagonists with the estrogen receptor and estrogen receptor independent pathways1617. Traditional medicinal plants like elder flower (sambuccus nigra) and parsley root (Petroselinum crispum) are known since centuries for their health benefits. Therefore, we chose them as plants for our investigations on breast cancer cells. In both publications extracts of the plants were prepared in different concentrations and analyzed by mass spectrometry. In the parsley root publication, breast cancer MCF7 and MCF12A cells were exposed with different PCE concentrations and incubated. After incubation, the effect of the different concentrations of PCE on MCF7 and MCF12A cells was analyzed using various tests. For cytotoxicity, metabolic activity and DNA synthesis performance LDH, MTT and BrdU proliferation assays were used respectively. In the elder flower publication, trophoblast tumor cell lines BeWo and JEG-3, as well as MCF7 breast cancer cells were exposed and then incubated at different EFE concentrations. Cells remaining untreated served as test controls. In supernatant cells estradiol production was tested with an ELISA method. Changes caused by EFE in ER/PR expression were analyzed by immunocytochemistry. It was demonstrated that the plants investigated contain a substantial amount of phytoestrogens. Moreover, both plants demonstrated anti-cancerogenic properties on breast cancer cell lines by means of receptor expression patterns and the inhibition of cell proliferation. If the effects observed are only caused by phytoestrogen action remains unclear. Further investigations on different breast cancer cells and with the isolated phytoestrogen substances is needed before proper clinical investigations can be planned.

• Dokumententyp: Dissertationen (Dissertation, LMU München)
• Keywords: phytoestrogens, breast cancer, proliferation, receptor expression, cell lines
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften ; 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• Fakultäten: Medizinische Fakultät
• Sprache der Hochschulschrift: Englisch
• Datum der mündlichen Prüfung: 4. März 2021
• 1. Bericht­erstatter:in: Jeschke, Udo