The Role of bFGF, IGF-I, PDGF and TGF-ß in the Expression of the Osteogenic Phenotype in Human Marrow-Derived Bone-Like Cells In Culture

Stobbe, David (2008): The Role of bFGF, IGF-I, PDGF and TGF-ß in the Expression of the Osteogenic Phenotype in Human Marrow-Derived Bone-Like Cells In Culture. Dissertation, LMU München: Faculty of Medicine

Abstract

Extensive studies have shown that undifferentiated cellular components of human bone marow are involved in the formation and renewal of bone tissue (osteogenic cascade). In this study, human mesenchymal stem cells were stimulated by bFGF, IGFI, PDGF-BB or TGF-ß1, in 2 different concentrations respectively (1 and 10 µg/mL). in vitro to assess their influence on differentiation to the osteoblastic phenotype. Thus human marrow could be used as a malleable basic element in bone engineering. The study clearly showed a stimulatory or suppressive effect of each of the respective growth factors. In particular the addition of TGF-ß1 directed the differentiation of mesenchymal stem cells of human bone marrow toward the osteoblastic phenotype.

• Item Type: Theses (Dissertation, LMU Munich)
• Keywords: bone, osteoblast, osteogenesis, bFGF, IGFI, PDGF-BB, TGF-ß1, mesenchymal stem cells
• Subjects: 600 Technology, Medicine > 610 Medical sciences and medicine; 600 Technology, Medicine
• Faculties: Faculty of Medicine
• Language: English
• Date of oral examination: 8. May 2008
• 1. Referee: Mutschler, Wolf
• Signature of the printed copy: 0700/UMD 12751