Kombination von Y-FISH und Immunfluoreszenz zum Nachweis eines „lymphoendothelialen“ Chimärismus in Sinuswandzellen von Lymphknoten nach gegengeschlechtlicher Knochenmarktransplantation

Bahlmann, Olaf (2007): Kombination von Y-FISH und Immunfluoreszenz zum Nachweis eines „lymphoendothelialen“ Chimärismus in Sinuswandzellen von Lymphknoten nach gegengeschlechtlicher Knochenmarktransplantation. Dissertation, LMU München: Medizinische Fakultät

Abstract

Sinus-lining cells (SLC) of lymph nodes (LN) are referred to as (immune-accessory) dendritic cells (DC) and as such, they are part of the Monocyte Macrophage System (MMS). Chimerism of various kinds of DC after sex-mismatched stem cell transplantation (SCT) has been reported, but data on chimerism of SLC have not been available so far. We examined 13 LN of different female individuals after sex-mismatched SCT, using a combined podoplanin immunostain and Y-FISH on single section slides. Twelve out of 13 LN contained chimeric cells, obviously predominantly lymphocytes, and eleven LN stained positive with podoplanin in SLC. In one of the LN simultaneously podoplanin-marked SLC revealed Y-chromosomal chimerism. Our results demonstrate that bone marrow derived cells are capable of regenerating SLC, at least at low frequency. These bone marrow derived cells are most likely hematopoietic stem cells (HSC) differentiating into blood monocytes which then form SLC, and after antigen contact SLC might, again, differentiate into FDC.

• Dokumententyp: Dissertationen (Dissertation, LMU München)
• Keywords: lymph node, sinus-lining cells, chimerism, sex-mismatched stem cell transplantation, combination of Y-FISH and immunofluorescence
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit ; 600 Technik, Medizin, angewandte Wissenschaften
• Fakultäten: Medizinische Fakultät
• Sprache der Hochschulschrift: Deutsch
• Datum der mündlichen Prüfung: 11. November 2007
• 1. Bericht­erstatter:in: Huss, Ralf