Harnessing Adeno Associated Virus (AAV) technology for the treatment of scarring

Rajendran, Vijayanand (2022): Harnessing Adeno Associated Virus (AAV) technology for the treatment of scarring. Dissertation, LMU München: Faculty of Medicine

Abstract

Deep skin wounds rapidly heal with scars and contractures by mobilizing extracellular matrix and cells from the fascia, deep beneath the dermal layer of the skin. Here, we demonstrate that Adeno-associated virus 8 (AAV8) is highly effective for transducing scar-forming fibroblasts in the fascia. p120 is up-regulated explicitly in scar-forming fibroblasts populations in response to injury. We demonstrate in animals that AAV8 mediated short hairpin RNA (ShRNA) silencing of p120, specifically in the fascia, reduces extracellular matrix (ECM) mobilization and enables scarless wound repair outcomes. Our findings demonstrate the potential for specifically targeting mechanisms of fascia mobilization with AAV8 vectors and the translational applications of this technology in modulating endogenous repair responses to restore the function of injured tissues in a scarless fashion.

• Item Type: Theses (Dissertation, LMU Munich)
• Keywords: Skin, Scar, p120, AAV, Fascia, Fibroblasts
• Subjects: 600 Technology, Medicine; 600 Technology, Medicine > 610 Medical sciences and medicine
• Faculties: Faculty of Medicine
• Language: English
• Date of oral examination: 3. June 2022
• 1. Referee: Rinkevich, Yuval
• Signature of the printed copy: 0700/UMD 21146