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Entwicklung einer sensitiven HPLC-Methode zur Bestimmung der Kontamination von Oberflächen mit Gemcitabin aus Wischproben
Entwicklung einer sensitiven HPLC-Methode zur Bestimmung der Kontamination von Oberflächen mit Gemcitabin aus Wischproben
Monitoring of work surfaces in health-care settings still shows detectable levels of antineoplastic drugs. The implementation of recent guidelines for the protection of healthcare workers’ safety and health has already resulted in decreased contamination levels of antineoplastic drugs, although compliance with the recommended practices is still inadequate. Hence, very sensitive methods for both environmental sampling and analysis are required to assess the contamination and therefore the risk of exposure of antineoplastic drugs such as gemcitabine in health-care settings. In the present study a high-performance liquid chromatography (HPLC) assay for the sensitive determination of gemcitabine in wipe samples was developed. For sample concentration and especially cleanup and purification a solid-phase extraction (SPE) protocol with appropriate buffer solutions was used. A limit of detection (LOD) of 0.091 μg/ml was obtained. To assess the developed analytical method, surface wipe sampling was performed at eight different work surfaces in health-care settings.
gemcitabine, HPLC, wipe sample, occupational medicine
Rosenkranz, Leonard
2020
English
Universitätsbibliothek der Ludwig-Maximilians-Universität München
Rosenkranz, Leonard (2020): Entwicklung einer sensitiven HPLC-Methode zur Bestimmung der Kontamination von Oberflächen mit Gemcitabin aus Wischproben. Dissertation, LMU München: Faculty of Medicine
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Abstract

Monitoring of work surfaces in health-care settings still shows detectable levels of antineoplastic drugs. The implementation of recent guidelines for the protection of healthcare workers’ safety and health has already resulted in decreased contamination levels of antineoplastic drugs, although compliance with the recommended practices is still inadequate. Hence, very sensitive methods for both environmental sampling and analysis are required to assess the contamination and therefore the risk of exposure of antineoplastic drugs such as gemcitabine in health-care settings. In the present study a high-performance liquid chromatography (HPLC) assay for the sensitive determination of gemcitabine in wipe samples was developed. For sample concentration and especially cleanup and purification a solid-phase extraction (SPE) protocol with appropriate buffer solutions was used. A limit of detection (LOD) of 0.091 μg/ml was obtained. To assess the developed analytical method, surface wipe sampling was performed at eight different work surfaces in health-care settings.