Decreased demand for olfactory periglomerular cells impacts on neural precursor cell viability in the rostral migratory stream

Gu, Song (2018): Decreased demand for olfactory periglomerular cells impacts on neural precursor cell viability in the rostral migratory stream. Dissertation, LMU München: Medizinische Fakultät

Abstract

The subventricular zone (SVZ) provides a constant supply of new neurons to the granule cell-layer of the olfactory bulb (OB) and new interneurons for the glomerular layer likely arise from the rostral migratory stream (RMS). Different studies have investigated the role of olfactory sensory input to neural precursor cell (NPC) turnover in the SVZ but it was not addressed if a reduced demand specifically for periglomerular neurons impacts on NPC-traits in the RMS. We here report that membrane type-1 matrix metalloproteinase (MT1-MMP) deficient mice have reduced complexity of the nasal turbinates, decreased sensory innervation of the OB, reduced numbers of olfactory glomeruli and reduced OB-size without alterations in SVZ neurogenesis. The rostral migratory stream (RMS) was fully preserved in MT1-MMP-deficient mice, but we detected an increase in cell death-levels in the distal RMS, as compared to controls. BrdU-tracking experiments showed that homing of NPCs specifically to the glomerular layer was reduced in MT1-MMP-deficient mice in contrast to controls while numbers of tracked cells remained equal in other OB-layers throughout all experimental groups. Altogether, our data show the demand for olfactory interneurons in the glomerular layer modulates cell turnover in the RMS, but has no impact on subventricular neurogenesis.

• Dokumententyp: Dissertationen (Dissertation, LMU München)
• Keywords: NPCs, SVZ, Neurogenesis, RMS, OB, MT1-MMP
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften ; 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• Fakultäten: Medizinische Fakultät
• Sprache der Hochschulschrift: Englisch
• Datum der mündlichen Prüfung: 29. November 2018
• 1. Bericht­erstatter:in: Glaß, Rainer