Ramanjaneyulu, Allam (2010): The role of cytosolic RNA and DNA recognition in systemic autoimmunity and immune complex glomerulonephritis: Cytosoli RNA and DNA recognition in autoimmunity. Dissertation, LMU München: Faculty of Medicine |
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Abstract
Non-CpG-DNA and 3P-RNA activated mesangial cells to produce inflammatory cytokines and type 1 interferons in TLR-independent manner. Both ligands induced similar gene expression patterns in mesangial cells. This data unravelled the existence of TLR-independent pathways and production of type1 interferons in mesangial cells. These results substantiate the idea that, immune complexes that are associated with nucleic acids can activate glomerular cells via TLR-independent manner, which leads to glomerular inflammation. Furthermore, exposure of non-CpG-DNA and 3P-RNA in MRLlpr/lpr mice aggravated the disease pathology in different manner. Even though 3P-RNA and non-CpG-DNA induced similar gene expression in mesangial cells but in vivo both ligands aggravated the disease in different fashion. 3P-RNA induced type I IFN signaling and decreased the number of regulatory T cells while non-CpG-DNA induced plasma cell expansion, lymphoproliferation and splenomegaly. However, both ligands induced the production of dsDNA autoantibodies and increased the glomerular deposition of IgG. These data suggest that viral 3P-RNA and non-CpG-DNA differently modulate autoimmunity but still both aggravate autoimmune tissue injury by activating non-immune cells at the tissue level
Item Type: | Theses (Dissertation, LMU Munich) |
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Keywords: | Autoimmunity,Lupus, TLRs, Cytosolic DNA, Cytosolic RNA |
Subjects: | 600 Technology, Medicine 600 Technology, Medicine > 610 Medical sciences and medicine |
Faculties: | Faculty of Medicine |
Language: | English |
Date of oral examination: | 20. May 2010 |
1. Referee: | Anders, Hans-Joachim |
MD5 Checksum of the PDF-file: | 9f93fc369b7e391ccc3b58923168d349 |
Signature of the printed copy: | 0700/UMD 13823 |
ID Code: | 11543 |
Deposited On: | 02. Jun 2010 13:03 |
Last Modified: | 24. Oct 2020 05:02 |