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Characterization of recombinant BVDV-2 vaccine prototypes based on packaged replicons and replication competent deletion mutants
Characterization of recombinant BVDV-2 vaccine prototypes based on packaged replicons and replication competent deletion mutants
Vaccination against Bovine Viral Diarrhea Virus plays a major role in the obligatory German control program decided in 2008. Both kinds of vaccines licensed today for the use in cattle have disadvantages: MLV vaccines concerning their safety and killed vaccines concerning efficacy. In the presented work, virus generated by transfection of conventional bovine cell lines with RNA derived from a new BVDV-2 full-length clone (Mischkale et al., 2009) was used to infect cattle. Its effects compared to the wild type strain were investigated. Further, two mutants derived from this BVDV-2 full-length cDNA clone were tested in a vaccination-challenge trial in cattle following two approaches of attenuation (pseudovirions and deletion of the Npro protein). Their safety and efficacy as vaccine candidates were investigated.
Bovine viral diarrhea virus type 2, vaccination, infectious pestivirus clone, pseudovirions, modified-live vaccine,
Zemke, Johanna
2010
English
Universitätsbibliothek der Ludwig-Maximilians-Universität München
Zemke, Johanna (2010): Characterization of recombinant BVDV-2 vaccine prototypes based on packaged replicons and replication competent deletion mutants. Dissertation, LMU München: Faculty of Veterinary Medicine
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Abstract

Vaccination against Bovine Viral Diarrhea Virus plays a major role in the obligatory German control program decided in 2008. Both kinds of vaccines licensed today for the use in cattle have disadvantages: MLV vaccines concerning their safety and killed vaccines concerning efficacy. In the presented work, virus generated by transfection of conventional bovine cell lines with RNA derived from a new BVDV-2 full-length clone (Mischkale et al., 2009) was used to infect cattle. Its effects compared to the wild type strain were investigated. Further, two mutants derived from this BVDV-2 full-length cDNA clone were tested in a vaccination-challenge trial in cattle following two approaches of attenuation (pseudovirions and deletion of the Npro protein). Their safety and efficacy as vaccine candidates were investigated.