Zemke, Johanna (2010): Characterization of recombinant BVDV-2 vaccine prototypes based on packaged replicons and replication competent deletion mutants. Dissertation, LMU München: Faculty of Veterinary Medicine |
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Abstract
Vaccination against Bovine Viral Diarrhea Virus plays a major role in the obligatory German control program decided in 2008. Both kinds of vaccines licensed today for the use in cattle have disadvantages: MLV vaccines concerning their safety and killed vaccines concerning efficacy. In the presented work, virus generated by transfection of conventional bovine cell lines with RNA derived from a new BVDV-2 full-length clone (Mischkale et al., 2009) was used to infect cattle. Its effects compared to the wild type strain were investigated. Further, two mutants derived from this BVDV-2 full-length cDNA clone were tested in a vaccination-challenge trial in cattle following two approaches of attenuation (pseudovirions and deletion of the Npro protein). Their safety and efficacy as vaccine candidates were investigated.
Item Type: | Theses (Dissertation, LMU Munich) |
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Keywords: | Bovine viral diarrhea virus type 2, vaccination, infectious pestivirus clone, pseudovirions, modified-live vaccine, |
Subjects: | 500 Natural sciences and mathematics 500 Natural sciences and mathematics > 590 Zoological sciences |
Faculties: | Faculty of Veterinary Medicine |
Language: | English |
Date of oral examination: | 13. February 2010 |
1. Referee: | Straubinger, Reinhard |
MD5 Checksum of the PDF-file: | 981112d7807cfa7ad63fe7995f5f9a1f |
Signature of the printed copy: | 0001/UMC 18594 |
ID Code: | 11471 |
Deposited On: | 31. May 2010 07:16 |
Last Modified: | 24. Oct 2020 05:06 |