Characterization of recombinant BVDV-2 vaccine prototypes based on packaged replicons and replication competent deletion mutants

Zemke, Johanna (2010): Characterization of recombinant BVDV-2 vaccine prototypes based on packaged replicons and replication competent deletion mutants. Dissertation, LMU München: Tierärztliche Fakultät

Abstract

Vaccination against Bovine Viral Diarrhea Virus plays a major role in the obligatory German control program decided in 2008. Both kinds of vaccines licensed today for the use in cattle have disadvantages: MLV vaccines concerning their safety and killed vaccines concerning efficacy. In the presented work, virus generated by transfection of conventional bovine cell lines with RNA derived from a new BVDV-2 full-length clone (Mischkale et al., 2009) was used to infect cattle. Its effects compared to the wild type strain were investigated. Further, two mutants derived from this BVDV-2 full-length cDNA clone were tested in a vaccination-challenge trial in cattle following two approaches of attenuation (pseudovirions and deletion of the Npro protein). Their safety and efficacy as vaccine candidates were investigated.

• Dokumententyp: Dissertation (Dissertation, LMU München)
• Keywords: Bovine viral diarrhea virus type 2, vaccination, infectious pestivirus clone, pseudovirions, modified-live vaccine,
• Themengebiete: 500 Naturwissenschaften und Mathematik ; 500 Naturwissenschaften und Mathematik > 590 Tiere (Zoologie)
• Fakultäten: Tierärztliche Fakultät
• Sprache der Hochschulschrift: Englisch
• Datum der mündlichen Prüfung: 13. Februar 2010
• 1. Bericht­erstatter/in: Straubinger, Reinhard