Characterization of recombinant BVDV-2 vaccine prototypes based on packaged replicons and replication competent deletion mutants

Zemke, Johanna (2010): Characterization of recombinant BVDV-2 vaccine prototypes based on packaged replicons and replication competent deletion mutants. Dissertation, LMU München: Faculty of Veterinary Medicine

Abstract

Vaccination against Bovine Viral Diarrhea Virus plays a major role in the obligatory German control program decided in 2008. Both kinds of vaccines licensed today for the use in cattle have disadvantages: MLV vaccines concerning their safety and killed vaccines concerning efficacy. In the presented work, virus generated by transfection of conventional bovine cell lines with RNA derived from a new BVDV-2 full-length clone (Mischkale et al., 2009) was used to infect cattle. Its effects compared to the wild type strain were investigated. Further, two mutants derived from this BVDV-2 full-length cDNA clone were tested in a vaccination-challenge trial in cattle following two approaches of attenuation (pseudovirions and deletion of the Npro protein). Their safety and efficacy as vaccine candidates were investigated.

• Item Type: Theses (Dissertation, LMU Munich)
• Keywords: Bovine viral diarrhea virus type 2, vaccination, infectious pestivirus clone, pseudovirions, modified-live vaccine,
• Subjects: 500 Natural sciences and mathematics; 500 Natural sciences and mathematics > 590 Zoological sciences
• Faculties: Faculty of Veterinary Medicine
• Language: English
• Date of oral examination: 13. February 2010
• 1. Referee: Straubinger, Reinhard
• Signature of the printed copy: 0001/UMC 18594