Armache, Karim (2005): Crystal structures of the complete 12-subunit RNA polymerase II and its subcomplex Rpb4/7, and modeling of RNA polymerases I and III. Dissertation, LMU München: Faculty of Chemistry and Pharmacy |
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Abstract
RNA polymerase II (Pol II) is the central enzyme, that synthetizes all mRNA in eukaryotic cells. In this work, I solved the structure of the complete, initiation-competent 12-subunit yeast RNA polymerase II at 3.8 Å. I also solved the structure of the Pol II subcomplex of Rpb4/7 alone at 2.3 Å resolution. These structures reveal the details of Pol II assembly from 12 subunits and give important insights into the initiation of transcription. The refined, atomic model of the complete 12-subunit Pol II enabled homology modeling of the two other nuclear RNA polymerases. In Pol I and Pol III, 65 % and 77 % of the Pol II fold are conserved, respectively. Together with a recent structure of a Pol II elongation complex, these results show that the basic mechanism of transcription applies also to the two other nuclear RNA polymerases
Item Type: | Theses (Dissertation, LMU Munich) |
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Subjects: | 500 Natural sciences and mathematics > 540 Chemistry and allied sciences 500 Natural sciences and mathematics |
Faculties: | Faculty of Chemistry and Pharmacy |
Language: | English |
Date of oral examination: | 17. June 2005 |
1. Referee: | Cramer, Patrick |
MD5 Checksum of the PDF-file: | 5b4093def077a11a1db4c340b1f2e79c |
Signature of the printed copy: | 0001/UMC 15196 |
ID Code: | 4915 |
Deposited On: | 23. Feb 2006 |
Last Modified: | 24. Oct 2020 09:45 |