Auswirkung von Stabilisatormedien auf den Nachweis des Influenza-A-Virus bei Schweinen (IAV) aus Kaustrickproben unter Laborbedingungen

Grau, Kristin (2025): Auswirkung von Stabilisatormedien auf den Nachweis des Influenza-A-Virus bei Schweinen (IAV) aus Kaustrickproben unter Laborbedingungen. Dissertation, LMU München: Tierärztliche Fakultät

Abstract

Aggregated samples such as oral fluids (OFs) display an animal friendly and time and cost-efficient sample type for swine Influenza A virus (swIAV) monitoring. However, further molecular and biological characterization of swIAV is of particular significance. The reportedly inferior suitability of aggregated samples for subtyping of swIAV presents a major drawback compared to nasal swabs, still considered the most appropriate sample type for this purpose (1). In addition, the viral load in the original sample, storage conditions and characteristics of different swIAV strains might further compromise the eligibility of aggregated samples for molecular detection and subtyping. Therefore, the present study aimed to evaluate the suitability of stabilizing media to minimize the degradation of viral RNA and thusincrease the detection and subtyping rate of swIAV by RT-qPCR in spiked OFs under different conditions (virus strain, storage temperature and viral load in the original sample) over a time span of 14 days.

• Dokumententyp: Dissertationen (Dissertation, LMU München)
• Keywords: swine, Influenza A Virus, subtyping, surveillance, stabilizing media, RTqPCR
• Themengebiete: 500 Naturwissenschaften und Mathematik ; 500 Naturwissenschaften und Mathematik > 590 Tiere (Zoologie)
• Fakultäten: Tierärztliche Fakultät
• Sprache der Hochschulschrift: Deutsch
• Datum der mündlichen Prüfung: 8. März 2025
• 1. Bericht­erstatter:in: Eddicks, Matthias