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Regulation of B cell development by posttranslational modification of Ebf1
Regulation of B cell development by posttranslational modification of Ebf1
Early B cell differentiation is regulated via a complex transcriptional network. Ebf1 is a central part of it, regulating around 3000 target genes associated with B cell function. Among these target genes, Ebf1 plays diverse roles to activate, repress or poise gene expression. Post-translational modification represents a potential explanation for these diverse roles. Specifically, phosphorylation of Ebf1 might contribute to the diverse functions of Ebf1 during B cell development. The hypothesis of this study is that phosphorylation of Ebf1 at S13 and S14 plays a role in B cell development, and the aim of this study is to clarify the role of phosphorylation on Ebf1 S13 and S14 in early B cell development. This study confirms the post-translational modification of endogenous Ebf1 via monoclonal antibodies, which only recognized the phosphorylated Ebf1-S13 and S14. We investigate the role of phosphorylated Ebf1-S13 and S14 in primary B cells. As a transcription factor, the molecular mechanism of Ebf1 is to bind to DNA and regulate the expression of target genes. Therefore, we analyse the biological and biochemical function of phosphorylated Ebf1-S13 and S14 in B cell development.
B cell differentiation, Ebf1, Posttranslational modification
Wang, Qiongman
2016
English
Universitätsbibliothek der Ludwig-Maximilians-Universität München
Wang, Qiongman (2016): Regulation of B cell development by posttranslational modification of Ebf1. Dissertation, LMU München: Faculty of Biology
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Abstract

Early B cell differentiation is regulated via a complex transcriptional network. Ebf1 is a central part of it, regulating around 3000 target genes associated with B cell function. Among these target genes, Ebf1 plays diverse roles to activate, repress or poise gene expression. Post-translational modification represents a potential explanation for these diverse roles. Specifically, phosphorylation of Ebf1 might contribute to the diverse functions of Ebf1 during B cell development. The hypothesis of this study is that phosphorylation of Ebf1 at S13 and S14 plays a role in B cell development, and the aim of this study is to clarify the role of phosphorylation on Ebf1 S13 and S14 in early B cell development. This study confirms the post-translational modification of endogenous Ebf1 via monoclonal antibodies, which only recognized the phosphorylated Ebf1-S13 and S14. We investigate the role of phosphorylated Ebf1-S13 and S14 in primary B cells. As a transcription factor, the molecular mechanism of Ebf1 is to bind to DNA and regulate the expression of target genes. Therefore, we analyse the biological and biochemical function of phosphorylated Ebf1-S13 and S14 in B cell development.