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Vergleichende Differenzierung von Clostridium spp. mittels biochemischer und molekularbiologischer Methoden sowie MALDI-TOF Massenspektrometrie
Vergleichende Differenzierung von Clostridium spp. mittels biochemischer und molekularbiologischer Methoden sowie MALDI-TOF Massenspektrometrie
The aim of this study was to examine whether the classical biochemical differentiation methods in relation to clostridial analysis can be replaced by newer molecular biological techniques such as real-time PCR and by MALDI-TOF MS. For this purpose, 118 potentially pathogenic Clostridium spp. isolates were compared at the Bavarian Health and Food Safety Authority using the above mentioned methods. On the basis of the obtained results a recommendation concerning the general approach to the detection of Clostridium spp. in the food industry has been elaborated.
Clostridium perfringens, Clostridium botulinum, Clostridium difficile, real-time PCR, MALDI-TOF MS
Ziegler, Julia Susanne
2013
Deutsch
Universitätsbibliothek der Ludwig-Maximilians-Universität München
Ziegler, Julia Susanne (2013): Vergleichende Differenzierung von Clostridium spp. mittels biochemischer und molekularbiologischer Methoden sowie MALDI-TOF Massenspektrometrie. Dissertation, LMU München: Tierärztliche Fakultät
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Abstract

The aim of this study was to examine whether the classical biochemical differentiation methods in relation to clostridial analysis can be replaced by newer molecular biological techniques such as real-time PCR and by MALDI-TOF MS. For this purpose, 118 potentially pathogenic Clostridium spp. isolates were compared at the Bavarian Health and Food Safety Authority using the above mentioned methods. On the basis of the obtained results a recommendation concerning the general approach to the detection of Clostridium spp. in the food industry has been elaborated.