Vergleichende Differenzierung von Clostridium spp. mittels biochemischer und molekularbiologischer Methoden sowie MALDI-TOF Massenspektrometrie

Ziegler, Julia Susanne (2013): Vergleichende Differenzierung von Clostridium spp. mittels biochemischer und molekularbiologischer Methoden sowie MALDI-TOF Massenspektrometrie. Dissertation, LMU München: Tierärztliche Fakultät

Abstract

The aim of this study was to examine whether the classical biochemical differentiation methods in relation to clostridial analysis can be replaced by newer molecular biological techniques such as real-time PCR and by MALDI-TOF MS. For this purpose, 118 potentially pathogenic Clostridium spp. isolates were compared at the Bavarian Health and Food Safety Authority using the above mentioned methods. On the basis of the obtained results a recommendation concerning the general approach to the detection of Clostridium spp. in the food industry has been elaborated.

• Dokumententyp: Dissertationen (Dissertation, LMU München)
• Keywords: Clostridium perfringens, Clostridium botulinum, Clostridium difficile, real-time PCR, MALDI-TOF MS
• Themengebiete: 500 Naturwissenschaften und Mathematik ; 500 Naturwissenschaften und Mathematik > 590 Tiere (Zoologie)
• Fakultäten: Tierärztliche Fakultät
• Sprache der Hochschulschrift: Deutsch
• Datum der mündlichen Prüfung: 9. Februar 2013
• 1. Bericht­erstatter:in: Märtlbauer, Erwin