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Haedke, Kristina (2008): Untersuchungen zur Diagnostik des Polyserositis-Komplexes beim Schwein. Dissertation, LMU München: Faculty of Veterinary Medicine
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Abstract

Investigations into the diagnosis of the polyserositis complex in swine The aim of this study was to evaluate the PCR test results from collective swab samples of serous membranes for genomic fragments of H. parasuis and M. hyorhinis regarding their associations and correlations with the pretreatment, clinical and pathological findings as well as the detection of PRRSV EU strain and PCV-2. For the classification of the method in reference to H. parasuis the PCR results were compared with the results of the bacteriological examination of the BALF. Furthermore the association between PRRSV and PCV-2 as well as the correlations between the weight of the animals and the detection of the agents, the clinical score and the detection of the agents and between PCV-2 and H. parasuis, M. hyorhinis as well as PRRSV EU strain were determined. After the admission of the preliminary report, in which particularly the state of treatment and vaccination regarding H. parasuis was inquired, 143 pigs were clinically examined. The results were evaluated on the basis of a score system. From 117 pigs under neuroleptanalgesia bronchoalveolar lavage fluid was taken and examined bacteriologically for H. parasuis. The animals were euthanized afterwards and subjected to a gross pathological and histopathological examination. In the course of the necropsy collective swabs of the serous membranes were taken from the surfaces of pleura, pericard and peritoneum and analysed via PCR for genomic fragments of H. parasuis and M. hyorhinis. Additionally lung tissue samples were taken from 102 animals for the molecular biological proof of genomic fragments and tissue samples from lungs and lymph nodes were taken from 105 animals for in-situ hybridisation. The results show that the detection of the H. parasuis and M. hyorhinis in the PCR was not associated significantly with a previous treatment. No significant relation was found between the analysing methods, PCR from the collective serosal swab and bacteriological examination of the BALF. The detection of genomic fragments of H. parasuis showed a significant association with the findings of the auscultation. Furthermore the evidence of the agent in the PCR significantly correlated with the presence of CNS symptoms. The evaluation of the state of nutrition and the filling degree of the tarsal joints did not correlate significantly with the PCR proof of H. parasuis. In contrast significant relations were shown between the proof of genomic fragments of M. hyorhinis and the occurence of kyphosis, the findings of the auscultation and the evaluation of the state of nutrition, but the proof of genomic fragments of M. hyorhinis did not significantly correlate with the filling degree of the tarsal joints as well as the presence of CNS symptoms. The results of the pathological investigation showed a significant association of M. hyorhinis with the diagnosis of a catarrhal-suppurative bronchopneumonia as well as a significant association of both agents with the diagnosis of a serositis without differenciation of the site and for the singular diagnosis of a pleuritis and pericarditis. The proof of M. hyorhinis and H. parasuis in the PCR was not significantly associated with peritonitis and additionally that of H. parasuis was not significantly associated with the diagnosis of a catarrhal-suppurative bronchopneumonia. Significant associations were calculated between the detection of the following agents: H. parasuis and M. hyorhinis, H. parasuis and PRRSV EU strain as well as M. hyorhinis and PRRSV EU strain. In contrast the association between the detection of H. parasuis and PCV-2, M. hyorhinis and PCV-2 as well as PRRSV EU strain and PCV-2 was not significant. Furthermore associations were significant for the weight of the animals and the proof of PCV-2 respectively PRRSV EU strain, for the clinical score und the proof of H. parasuis respectively PCV-2 as well as for PCV-2 und PRRSV EU strain. In conclusion the sampling by collective dry swabs of the serous skins and the following PCR examination for genomic fragments is a meaningful method for the diagnosis of diseases, which are accompanied by polyserositis. With this procedure the proof of the agents can be carried out successfully in animals with clinical signs as well as in animals which are already pretreated with antibiotics. For H. parasuis and M. hyorhinis, both causal agents of polyserositis, a significant association was calculated. Both agents are capable of causing clinical signs and pathological findings associated with polyserositis. The disease induced by H. parasuis is called Glaesser´s disease, in reference to M. hyorhinis as Mycoplasma Polyserositis. Due to the existing association of both agents and the accompanied clinical and pathological signs it is reasonable to use the term “Polyserositis Complex”.