| Poeck, Hendrik (2006): Der Einfluss von plasmazytoiden dendritischen Zellen und immunstimulatorischer DNA auf B-Zellen des humanen Immunsystems. Dissertation, LMU München: Faculty of Medicine |
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Poeck_Hendrik.pdf 5Mb |
Abstract
It has been reported that interferon (IFN-) enhances humoral immunity and that dendritic cells of the myeloid lineage promote B-cell differentiation. Here we studied whether the plasmacytoid dendritic cell (PDC), a subset of dendritic cells specialized for the production of IFN-, is involved in regulating B-cell differentiation and immunoglobulin production. The recently identified class of CpG oligonucleotides (CpG-C) was used to activate both B cells and PDCs via Toll-like receptor 9 (TLR9). The presence of PDCs synergistically enhanced CD86 expression, cytokine production (interleukin 6 [IL-6], tumor necrosis factor , and IL-10) and plasma cell differentiation of isolated human peripheral blood B cells stimulated through CpG-C and B-cell antigen receptor (BCR) ligation. This stimulation protocol was sufficient to drive purified naive B cells into IgM-producing plasma cells and to trigger IgG synthesis in memory B cells. PDCs contributed to B-cell activation via IFN- secretion. Up-regulation of TLR9 on B cells was not involved. These results demonstrate that CpG-stimulated PDCs induce plasma cell differentiation in naive and memory B cells in the absence of T-cell help, providing an explanation for the excellent activity of CpG oligonucleotides as a humoral vaccine adjuvant. (Blood. 2004;103:3058-3064)
| Item Type: | Thesis (Dissertation, LMU Munich) | |
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| Keywords: | B cell, PDC, TLR, CpG oligonucleotides, adaptive immunity | |
| Subjects: | 600 Technology, Medicine > 610 Medical sciences and medicine 600 Technology, Medicine | |
| Faculties: | Faculty of Medicine | |
| Language: | German | |
| Date Accepted: | 6. July 2006 | |
| 1. Referee: | Endres, Stefan | |
| Persistent Identifier (URN): | urn:nbn:de:bvb:19-55599 | |
| MD5 Checksum of the PDF-file: | 4646796f33240a7f3074e66d447e0a27 | |
| Signature of the printed copy: | 0700/UMD 11667 | |
| ID Code: | 5559 | |
| Deposited On: | 31. Jul 2006 | (UTC) |
| Last Modified: | 16. Oct 2012 07:59 | (UTC) |