Crystal structures of the complete 12-subunit RNA polymerase II and its subcomplex Rpb4/7, and modeling of RNA polymerases I and III

Armache, Karim (2005): Crystal structures of the complete 12-subunit RNA polymerase II and its subcomplex Rpb4/7, and modeling of RNA polymerases I and III. Dissertation, LMU München: Faculty of Chemistry and Pharmacy

Abstract

RNA polymerase II (Pol II) is the central enzyme, that synthetizes all mRNA in eukaryotic cells. In this work, I solved the structure of the complete, initiation-competent 12-subunit yeast RNA polymerase II at 3.8 Å. I also solved the structure of the Pol II subcomplex of Rpb4/7 alone at 2.3 Å resolution. These structures reveal the details of Pol II assembly from 12 subunits and give important insights into the initiation of transcription. The refined, atomic model of the complete 12-subunit Pol II enabled homology modeling of the two other nuclear RNA polymerases. In Pol I and Pol III, 65 % and 77 % of the Pol II fold are conserved, respectively. Together with a recent structure of a Pol II elongation complex, these results show that the basic mechanism of transcription applies also to the two other nuclear RNA polymerases

• Item Type: Thesis (Dissertation, LMU Munich)
• Subjects: 600 Natural sciences and mathematics > 540 Chemistry and allied sciences; 600 Natural sciences and mathematics
• Faculties: Faculty of Chemistry and Pharmacy
• Language: English
• Date Accepted: 17. June 2005
• 1. Referee: Cramer, Patrick
• Persistent Identifier (URN): urn:nbn:de:bvb:19-49156
• Signature of the printed copy: 0001/UMC 15196