Inaktivierung des murinen Gens des Hyperpolarisations-aktivierten Zyklonukleotid-gesteuerten Kationenkanals Typ Drei (HCN3)

Abstract

The hyperpolarization-activated, cyclic-nucleotide-gated cation channel (HCN) familiy comprises four members. The channels play a role in the formation of rhythmic activity of heart and brain. In contrast to the other three members of the HCN channel family, there are only a few studies of tissue distribution and electrophysiological properties of HCN3 so far. Expression has been reported at low levels, but throughout the brain, in some other tissues such as retina, olfactory epithelium and recently in the heart. In order to study the physiological relevance of HCN3 expression, we generated HCN3-deficient mouse lines by gene targeting and homologous recombination: a complete Knockout, a complete Knockout expressing the reporter gene lacZ instead of HCN3 and a conditional Knockout using the Cre-loxP system to study spatial and temporal functions of this pacemaker channel. The functional characterization included behavioural studies and heart physiology.

• Item Type: Thesis (Dissertation, LMU Munich)
• Keywords: HCN3, knockout, ion channel, transgenic animal
• Dewey Decimal Classification: 600 Natural sciences and mathematics; 600 Natural sciences and mathematics > 540 Chemistry and allied sciences
• Faculties: Faculty of Chemistry and Pharmacy
• Language: German
• Date Accepted: 09. December 2004
• 1. Referee: Biel, Martin
• Persistent Identifier (URN): urn:nbn:de:bvb:19-29398
• Signature of the printed copy: 0001/UMC 14248